CEE2300HW5 help - CEE2300B / Konstantinidis / Homework 2...

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CEE2300B / Konstantinidis / Homework 2 Name: _________________________________________ Due date: Wednesday, April 7 th 1. If one starts with 10,000 bacterial cells in a culture that has a generation time of 2 hours, how many cells will be in the culture after 4, 24, and 48 hours? What is the total weight of the cells at each time point? 10 points Answer A typical bacterial cell weights one trillionth of a gram, i.e., 1.0 * 10 -12 g, (see slide on lecture #6). So, 4*10 4 * (1.0*10 -12 ) = 4*10 -8 g. 4.1*10 7 * (1.0*10 -12 ) = 4.1*10 -5 g. 1.68*10 11 * (1.0*10 -12 ) = 0.168g. Notice how fast bacterial biomass grows. 2. The following data were collected using a bacterial culture during growth in a defined medium containing toluene as a sole source of carbon and energy. Using these data, draw a growth curve, identify the different phases of growth, and determine the doubling time during exponential growth. 10 points Time (hours) Number of cells 0 2.2E+04 1.2 2.2E+04 2.2 2.2E+04 3.2 2.2E+04 4.2 1.5E+05 5.2 1.5E+07 6.2 8.1E+07 7.2 3.2E+08
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8.2 5.5E+08 9.2 8.0E+08 10.2 1.0E+09 11.2 1.7E+09 12.2 2.1E+09 13.2 2.3E+09 17.2 2.5E+09 32.2 6.3E+09 60.2 3.8E+09 84.2 1.9E+09 Answer
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3. List and define the two ways that all organisms use to capture (as ATP) the energy generated from the oxidation of organic compounds (food). Describe briefly the proton motive force and how it is created (use a simple diagram). 10 points Answer The two ways that energy is captured are: i) Oxidative (electron transport) phosphorylation , i.e., the nonphototrophic production of adenosine triphosphte (ATP) at the expense of a proton motive force formed by electron transport. ii)
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This note was uploaded on 10/05/2011 for the course CEE 2300 taught by Professor Spain during the Spring '08 term at Georgia Institute of Technology.

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CEE2300HW5 help - CEE2300B / Konstantinidis / Homework 2...

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