LAST QUIZ READ - A Bacterium That Can Grow by Using Arsenic...

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/ / 2 December 2010 / Page 1 / 10.1126/science.1197258 Life is mostly composed of the elements carbon, hydrogen, nitrogen, oxygen, sulfur and phosphorus. Although these six elements make up nucleic acids, proteins and lipids and thus the bulk of living matter, it is theoretically possible that some other elements in the periodic table could serve the same functions. Here we describe a bacterium, strain GFAJ-1 of the Halomonadaceae, isolated from Mono Lake, CA, which substitutes arsenic for phosphorus to sustain its growth. Our data show evidence for arsenate in macromolecules that normally contain phosphate, most notably nucleic acids and proteins. Exchange of one of the major bio-elements may have profound evolutionary and geochemical significance. Biological dependence on the six major nutrient elements carbon, hydrogen, nitrogen, oxygen, sulfur, and phosphorus is complemented by a selected array of other elements, usually metal(loid)s present in trace quantities that serve critical cellular functions, such as enzyme co-factors ( 1 ). There are many cases of these trace elements substituting for one another. A few examples include the substitution of tungsten for molybdenum and cadmium for zinc in some enzyme families ( 2, 3 ) and copper for iron as an oxygen-carrier in some arthropods and mollusks ( 4 ). In these examples and others, the trace elements that interchange share chemical similarities that facilitate the swap. However, there are no prior reports of substitutions for any of the six major elements essential for life. Here we present evidence that arsenic can substitute for phosphorus in the biomolecules of a naturally- occurring bacterium. Arsenic (As) is a chemical analog of phosphorus (P), which lies directly below P on the periodic table. Arsenic possesses a similar atomic radius, as well as near identical electronegativity to P ( 5 ). The most common form of P in biology is phosphate (PO 4 3- ), which behaves similarly to arsenate (AsO 4 3- ) over the range of biologically relevant pH and redox gradients ( 6 ). The physico-chemical similarity between AsO 4 3- and PO 4 3- contributes to the biological toxicity of AsO 4 3- because metabolic pathways intended for PO 4 3- cannot distinguish between the two molecules ( 7 ) and arsenate may be incorporated into some early steps in the pathways [( 6 ) and refs therein]. However, it is thought that downstream metabolic processes are generally not compatible with As-incorporating molecules because of differences in the reactivities of P- and As-compounds ( 8 ). These downstream biochemical pathways may require the more chemically stable P-based metabolites; the lifetimes of more easily hydrolyzed As-bearing analogs are thought to be too short. However, given the similarities of As and P, and by analogy
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This note was uploaded on 10/06/2011 for the course BIO 313 taught by Professor Rodermel during the Spring '11 term at Iowa State.

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LAST QUIZ READ - A Bacterium That Can Grow by Using Arsenic...

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