DNA technology and variation

DNA technology and variation - Download 13 DNA Technology...

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Download 13 DNA Technology & Variation Principles of Biology BIOL 1543 Greig Roberts, DDS Office: SCEN 417 E-Mail: groberts@uark.edu
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2 DNA and Crime Scene Investigations Many violent crimes go unsolved for lack of enough evidence. If biological fluids are left at a crime scene DNA can be isolated from them. DNA fingerprinting is a set of laboratory procedures that determines with near certainty whether two samples of DNA are from the same individual.
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3 Bacterium Bacterial chromosome Plasmid 1 Plasmid isolated 3 Gene inserted into plasmid 2 DNA isolated Cell containing gene of interest DNA Gene of interest Recombinant DNA (plasmid) 4 Plasmid put into bacterial cell Recombinant bacterium 5 Cell multiplies with gene of interest Copies of protein Copies of gene Clone of cells Gene for pest resistance inserted into plants Gene used to alter bacteria for cleaning up toxic waste Protein used to dissolve blood clots in heart attack therapy Protein used to make snow form at higher temperature Recombinant bacteria and gene cloning Researchers can insert desired genes into bacteria. Enzymes are used to “cut and paste” DNA. The “recombinant” bacteria multiply and copy the inserted genes.
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4 Cell nucleus DNA of eukaryotic gene Exon Intron Exon Intron Exon 1 Transcription 2 RNA splicing
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DNA technology and variation - Download 13 DNA Technology...

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