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Viruses and Cloning

Viruses and Cloning - Download 9 Viruses and Cloning...

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Download 9 Viruses and Cloning Principles of Biology BIOL 1543 Greig Roberts, DDS Office: SCEN 417 E-Mail: [email protected]
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2 Viruses and genetic material Viral DNA may become part of the host chromosome. Viruses are genes packaged in protein. Head Tail Tail fiber DNA 3 0 0 , 0 0 0 × A “phage” “A piece of nucleic acid  surrounded by bad  news…”  - Nobel laureate Peter  Medawar
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3 When phage DNA enters a lytic cycle inside a bacterium, it is replicated, transcribed, and translated. The new viral DNA and protein molecules then assemble into new phages, which burst from the host cell. The lytic cycle Phage attaches to bacterial cell. Phage injects DNA. Phage DNA directs host cell to make more phage DNA and protein parts. New phages assemble. Cell lyses and Releases new phages.
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4 In the lysogenic cycle , phage DNA inserts into the host chromosome and is passed on to generations of daughter cells. Much later, it may initiate phage production. The lysogenic cycle
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5 Lysogenic bacterium replicates the prophage in cell division Phage DNA inserts into the bacterial chromosome by recombination New phage DNA and proteins are synthesized Phages assemble Cell lyses, releasing phages Phage Attaches to cell Phage DNA Phage injects DNA Many cell divisions Prophage Lytic cycle Lysogenic cycle OR Bacterial chromosome Phage DNA circularizes Phage reproductive cycles
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6 Viruses and disease
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