Effect of Salt Concentration of Beta-lactamase Activity

Effect of Salt Concentration of Beta-lactamase Activity -...

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The Effect of Salt Concentration on the Activity of ß-lactamase By: Shivani Patel Nick Sommariva Annie Tang Pamela Pimentel
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ß-lactamase Hydrolyzes the ß-lactam rings Medical Significance
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Protein Folding Hydrophobic interactions and hydrogen bonding Electrostatic interactions Salt Bridges
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Experiment Hypothesis: Increasing or decreasing salt concentration will effect ß- lactamase absorbance Prediction: If the salt concentration is too high or too low, the ß-lactamase absorbance will be closer to the extremes of the spectrum
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Experiment Independent Variables: salt concentration Dependent Variable: absorbance Control Variables: same spectrophotometer, same temperature, same fraction of ß- lactamase Control Experiment: Sample without salt Sample without salt or ß-lactamase
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Protocol Make sure to zero your spectrophotometer For the first control, add 1 mL of nitrocefin, 20 μL of sodium phosphate buffer, 122 μL of 5M salt solution, and 78 μL of water (creating a .5M
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This note was uploaded on 10/17/2011 for the course ECON 101 taught by Professor Dezhbakhsh during the Fall '07 term at Emory.

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Effect of Salt Concentration of Beta-lactamase Activity -...

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