gen380F11StLect9

gen380F11StLect9 - GENETICS 380 LECTURE 9 Gene Linkage in...

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http://www.cellimagelibrary.org/images/10086 GENETICS 380 LECTURE 9: Gene Linkage in Prokaryotes
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Bacteria as a model organism for genetics - cheap - easy and fast to grow in large numbers - can be studied in pure cultures - haploid, so dominant vs recessive is not an issue - phenotype = genotype E. coli. Scanning E.M. S. Owens; Michigan State University
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Different traits allow us to study genetic variation in bacteria - sensitive or resistant to infection by bacteriophage - sensitive or resistant to antibiotics - ability to grow on specific media
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Growing Bacteria - in liquid culture or on solid media in petri dish - bacteria wild-type for all growth requirements - sugar (carbon source) - various ions - can survive on minimal media - synthesize all necessary organic compounds - some bacteria can’t synthesize all necessary organics - must be grown on complete media - supplemented with needed compound(s)
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Recombination in bacteria - Joshua Lederberg and Edward Tatum (1946) - studied 2 auxotrophic E. coli strains - strain Y24: - strain Y10: requires phenylalanine (phe) cysteine (cys) biotin (bio) requires threonine (thr) leucine (leu) thiamine (thi)
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no growth no growth growth Y10 + Y24 Recombination in bacteria Fig. 8-10; p.206
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