labweek2 - slant and broth INCUBATE M. Luteus @ 25 o C and...

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Laboratory Week #2 Read 1-2 MEDIA Media Prep Media must be sterile General purpose media Selective media Differential media Undefined or complex media Synthetic or chemically defined Forms of media Nutrient broth Nutrient agar Slant Deep Plate Lab 1-3 ASEPTIC TRANSFER AND INOCULATION Aseptic- Inoculating instruments Sterilize using incineration (dry) Loop Needle Pre-sterilized (in wrappers) Cotton swabs Transfer pipettes
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Vortex mixer- resuspension of organisms LAB EXERCISE 1-3 Transfer from broth, slant, agar plate a. E coli slant slant and broth b. M luteus slant slant and broth c. M. luteus broth slant and broth d. M. luteus plate
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Unformatted text preview: slant and broth INCUBATE M. Luteus @ 25 o C and E. coli @ 37 o C LAB EXERCISE 1-4 STREAK PLATE ISOLATION Mixed culture- one or more species Pure culture- single species Use four-way quadrant streak to isolate a pure culture from a mixed one Pure organism will give rise to a visible __________ LAB Exercise: a. From a mixed culture of S.marcescens and S. epidermidis perform a four way quadrant streak b. From an environmental source, dip a sterile pipette into a tt of distilled water, obtain a specimen. Use the swab for the first quadrant and use the loop for the rest....
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This note was uploaded on 10/18/2011 for the course MCB 2010 taught by Professor Elizabethingram during the Fall '11 term at Valencia.

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labweek2 - slant and broth INCUBATE M. Luteus @ 25 o C and...

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