labweek4 - LAB EXERCISES WEEK 4 Differential Stains allow...

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LAB EXERCISES WEEK # 4 Differential Stains – allow us to detect differences in parts of an organism or differences between organisms Gram stain - differences in gram+ and gram- organisms Acid- fast stain - Some organisms are gram variable and thus do not always stain the same in a gram stain. The genus Mycobacterium contains organisms that are gram variable. These organisms will be identified using the Acid-fast technique of either Ziehl-Nielson (ZN) or Kinyoun staining method. Their high mycolic acid content (waxy) make this stain ideal for identification of the organism Spore stain – Bacillus and Clostridium are the two most common genera of spore-producing organisms. A spore will form when conditions are not hospitable for the vegetative state to grow due to physical conditions or lack of nutrients. Exercise 3-6 The Gram Stain 1.Prepare 4 clean slides and allow them to air dry. 2. Make 3 identical slides placing both E coli and M luteus next to each other on the lower half of the slide. Air dry and then heat-fix. 3. Gram stain according to the procedure found in the lab
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This note was uploaded on 10/18/2011 for the course MCB 2010 taught by Professor Elizabethingram during the Fall '11 term at Valencia.

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labweek4 - LAB EXERCISES WEEK 4 Differential Stains allow...

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