problem1_2011 - c c = a [1 + ( 1 k 1 k 2 ) k 2 e k 1 t k 1...

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
Biochemistry 101a Problem Set 1 1. Show graphically (i)the change in concentration of [B] over time and (ii) the dependence of the reaction velocity from the substrate concentration [A] for the following reactions: a) A k 1 ί ί B b) A + A k 1 ί ί B c) A + E k ί ί k 1 ί ί AE k 2 ί ί B for [A] >> [K m ] E= enzyme 2. Derive the equations of dependence of [A] and [B] with respect to time for the a reversible reaction: A k 1 ί ί k 1 ί ί B And show it graphically for k 1 = 2x k -1 3. For the follow reaction A k 1 ί ί B k 2 ί ί C the concentration of [C] is dependent on t as follows (you can derive it if you want):
Background image of page 1
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: c c = a [1 + ( 1 k 1 k 2 ) k 2 e k 1 t k 1 e k 2 t ( ) ] What happens if k 1 >> k 2 or k 1 <<k 2 ? 4. Draw the titration curves for all functional groups for the following peptide: + H 3 N- Asp-Ala-Trp-His-Lys-Arg-Pro-Phe-CONH 2 Which pKa value would you expect for this peptide (qualitatively)? This peptide is a substrate for chymotrypsin. How would you determine (easy, in the lab) the concentration of your stock solution of peptide for an enzyme assay?...
View Full Document

This note was uploaded on 10/06/2011 for the course AAAS 101 taught by Professor Blanc during the Winter '08 term at Brandeis.

Ask a homework question - tutors are online