Unformatted text preview: c c = a [1 + ( 1 k 1 − k 2 ) k 2 e − k 1 t − k 1 e − k 2 t ( ) ] What happens if k 1 >> k 2 or k 1 <<k 2 ? 4. Draw the titration curves for all functional groups for the following peptide: + H 3 N- Asp-Ala-Trp-His-Lys-Arg-Pro-Phe-CONH 2 Which pKa value would you expect for this peptide (qualitatively)? This peptide is a substrate for chymotrypsin. How would you determine (easy, in the lab) the concentration of your stock solution of peptide for an enzyme assay?...
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- Winter '08
- Enzyme, Chemical reaction, k2, k−1Ⱥ 0F AE Ⱥ 0F