problem1_2011

problem1_2011 - c c = a [1 + ( 1 k 1 k 2 ) k 2 e k 1 t k 1...

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Biochemistry 101a Problem Set 1 1. Show graphically (i)the change in concentration of [B] over time and (ii) the dependence of the reaction velocity from the substrate concentration [A] for the following reactions: a) A k 1 ί ί B b) A + A k 1 ί ί B c) A + E k ί ί k 1 ί ί AE k 2 ί ί B for [A] >> [K m ] E= enzyme 2. Derive the equations of dependence of [A] and [B] with respect to time for the a reversible reaction: A k 1 ί ί k 1 ί ί B And show it graphically for k 1 = 2x k -1 3. For the follow reaction A k 1 ί ί B k 2 ί ί C the concentration of [C] is dependent on t as follows (you can derive it if you want):
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Unformatted text preview: c c = a [1 + ( 1 k 1 k 2 ) k 2 e k 1 t k 1 e k 2 t ( ) ] What happens if k 1 >> k 2 or k 1 <<k 2 ? 4. Draw the titration curves for all functional groups for the following peptide: + H 3 N- Asp-Ala-Trp-His-Lys-Arg-Pro-Phe-CONH 2 Which pKa value would you expect for this peptide (qualitatively)? This peptide is a substrate for chymotrypsin. How would you determine (easy, in the lab) the concentration of your stock solution of peptide for an enzyme assay?...
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This note was uploaded on 10/06/2011 for the course AAAS 101 taught by Professor Blanc during the Winter '08 term at Brandeis.

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