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Unformatted text preview: B) Chromatography 1) Obtain three chromatography plates and three test tubes. 2) Pour acetone into one test tube, hexane into another, and a 1:1 ratio of acetone and hexane into the third. Make sure the solvent in the test tube does not exceed a height of about 1cm. 3) Draw a line on each of the chromatography strips about a little over 1cm from the bottom. 4) Using capillary action, put a dot of the spinach extract, just above the line you just drew, on each chromatography strip. 5) Place one strip in each test tube and cover the top with parafilm. 6) Let the strips sit in the solvent for 10 minutes. 7) Remove the strips and let them dry. 8) Calculate the Rf values....
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This note was uploaded on 10/10/2011 for the course PHIL 160 taught by Professor Coakley during the Spring '11 term at University of Arizona- Tucson.
- Spring '11