PP midterm to SB

PP midterm to SB - Catalase, Oxidase, Amylase, Cellulase,...

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Catalase, Oxidase, Amylase, Cellulase, Caseinase, and Gelatinase BIOL 2051 General Microbiology Lab 13 3/15/10
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Miscellanea Midterms
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Introduction to Enzymes Morphology (shape, size, arrangement) vs. physiology (metabolic capabilities) Enzymes proteins that act as biological catalysts. Convert substrates to products. Named by location Exoenzymes/Extracelluar Endoenzymes Named by when enzyme is produced Inducible/Adaptive Constitutive
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New! Expt. 21 – Catalase Production Enzyme that breaks down hydrogen peroxide into water and oxygen: Hydrogen peroxide is a byproduct of aerobic metabolism. Hydrogen peroxide is toxic so it must be broken down. Catalase test is used to distinguish between organisms, especially Staphylococcus and Streptococcus . Streptococcus, Lactococcus, Enterococcus are catalase negative (fermentative or anaerobic bacteria) Staphylococcus and Micrococcus are catalase positive (aerobic bacteria) 2 H 2 O 2 2 H 2 O + O 2
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Expt. 21 Procedure (page 122) Add drop of H 2 O 2 to each side of a glass slide. Mix loop of Streptococcus mutans with one drop and Staphylococcus epidermidis with the other drop Look for bubbles (oxygen) which indicates positive catalase test result. + -
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New! Expt. 22 – Oxidase Production Purpose : to see cytochrome c oxidase produced by microorganisms Oxidase tests are used to differentiate medically relevant genera Vibrio and Pseudomonas (oxidase positive) from Enteric bacteria like Escherichia and Salmonella (oxidase negative) Neisseria and Moraxella (oxidase positive) from Acinetobacter (oxidase negative)
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Oxidase enzymes are made by cells that do respiration. Cytochrome oxidase transfers electrons to O 2 to form H 2 O or H 2 O 2 as the final electron transfer in electron transport of aerobic respiration. There are different types of cyt oxidases, of which cyt c oxidase is one. Shown in the figure is cyt bo 3 oxidase in E.coli . Electron transport in E. coli
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Expt. 22 Procedure (page 126) Label 1 TSA plate (per pair) Use your inoculating loop inoculate plate with a line of Pseudomonas putida and a line of Escherichia coli on separate sides of the plate. Incubate plate on the room temp shelf. Pp Ec Pp Ec
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New ! Expt. 23 – The Hydrolysis of Polysaccharides: Amylase Production Carbohydrates (CH 2 O) n Monosaccharide ex. Glucose–single molecule of a sugar Polysaccharide ex. cellulose, starch–each are polymers of glucose Amylase enzyme that hydrolyzes starch into smaller subunits (glucose) to be transported into a cell Microbially produced amylases have commercial and industrial uses food, fermentation, brewing, distilling, textiles, paper, etc.
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To test for microbes ability to produce amylase, inoculate organism onto starch agar (nutrient agar plus starch).
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This note was uploaded on 10/11/2011 for the course BIOL 2051 taught by Professor Brininstool during the Spring '07 term at LSU.

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PP midterm to SB - Catalase, Oxidase, Amylase, Cellulase,...

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