Meyer_Powerpoints_Before_Midterm

Meyer_Powerpoints_Before_Midterm - BIOL 2051 Microbiology...

Info iconThis preview shows pages 1–10. Sign up to view the full content.

View Full Document Right Arrow Icon
BIOL 2051: Microbiology Lab Sections 4 & 5 My name: Josh Meyer Email: [email protected] Office Hours: By Appointment Office: A647 Life Sciences Building Annex Lab Coordinator: Dr. Ginger Brininstool Office: 602 Life Sciences Building Phone: 578-4089 Email: [email protected]
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Syllabus Available on your Moodle page Lab manual Must purchase new one at bookstore Taxonomy & Systematics (pp. v - vi ) Lab Safety & Protocols (p. vii - ix ) Lab rules (pp. xi - xiii ) Discard rules (p. xv ) Lab manual will be graded twice during midterm & final Lab Supplies & Attire Lab Kit Safety glasses Disposable gloves Close-toed shoes Lab coat (optional)
Background image of page 2
Grading - 170 points: quizzes (19 total, drop two) - 100 points: midterm - 100 points: final - 29 points: lab manual checks - 20 points: unknowns project (experiment 32) - 15 points: microscope care & lab technique Attendance is mandatory and will affect your grade Lab manual questions and data: Keep up with them Two lab manual checks Any extra lab materials will be posted on Moodle
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Vesphene Use to disinfect benches Lab Drawers Slide box, clothespin, inoculating loops, lens paper, bibulous paper, slides Do not store personal items here! Cart Top is material needed for experiments Bottom is discarded tubes Discard Refer to posters or ask if you are not sure where something should be discarded Incubators 37 ° C incubator Room temperature shelf (blue test tube racks also go here)
Background image of page 4
Medium – mixture of nutrients in which a micro-organism can grow (pl.: media) Solid medium contains agar (from red algae) Melts @ 100 ° C, - Solidifies @ 40-42 ° C Types Plate - isolation Slant - maintenance Broth - liquid Deep – anaerobic growth Media Prep. & Sterilization—Expt. 1
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Glucose 5.0g Ammonium phosphate 1.0g (NH 4 H 2 PO 4 ) Sodium chloride 5.0g (NaCl) Magnesium sulfate 0.2g (MgSO 4 ·7 H 2 O) Water 1.0L Glucose 15.0g Yeast extract 5.0g Sodium chloride 5.0g Peptone 5.0g Water 1.0L Defined Complex Complex vs. Defined medium Complex medium – composed of digests of chemically undefined substances such as yeast and meat extracts Defined medium – medium whose precise chemical composition is known
Background image of page 6
Sterilization : killing or removal of all living organisms Autoclave: 121 ° C (for media, glass) Dry heat: flaming, hot air (for loops, glass) Filtration: for heat-sensitive liquids (antibiotics) Radiation: UV, gamma rays (for surfaces) Cold sterilization: ethylene oxide (for heat- sensitive materials)
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Labeling Write your initials, section number, date, and experiment number Petri dishes Use wax pencil or sharpie to write on bottom of Petri dish Test tubes Use the white labels Do not write directly on tubes GMB, sec.13, 9/12/09, exp. #7 GMB, sec.12, 9/12/09, exp. #7
Background image of page 8
Exp 2 - Culturing from the Environment Microorganisms found everywhere - land, sea, air, any surface and any condition Majority of these are NOT pathogenic (harmful) to humans Purpose
Background image of page 9

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 10
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

Page1 / 154

Meyer_Powerpoints_Before_Midterm - BIOL 2051 Microbiology...

This preview shows document pages 1 - 10. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online