Lecture 4-Microbial nutrition colored

Lecture 4-Microbial nutrition colored - 1 Microbial...

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Unformatted text preview: 1 Microbial Microbial Nutrition Nutrition Bio3124 Lecture # 4 Lecture outline Reading: Ch. 4 (up to page 130) Lecture topics Nutritional requirements Nutritional classes of microorganisms Nutrient uptake mechanisms Culturing bacteria and culture media Culturing and pure colony isolation methods Nutritional Requirements Nutritional Requirements Nutrients are substances required for biosynthesis of macromolecules, energy production and growth macroelements (macronutrients) required in relatively large amounts C, H, N, O, P, S and K, Mg, Fe and Ca micronutrients (trace elements) Mn, Zn, Co, Mo, Ni, and Cu required in trace amounts, used as cofactors by enzymes often supplied in water or in media components 2 Mystery Behind Life Mystery Behind Life Molecules of life are formed through reductive pathway that requires: Source of electrons and protons Source of energy A carbon source at oxidized state A carbon source at oxidized state Process: energy is used to release electrons from an inroganic/organic source Transfer onto a carbon containing molecule The reduced form of carbon is used to build new macrmolecular derivatives Growth Factors Growth Factors Are organic compounds essential cell components (or their precursors) that the cell cannot synthesize must be supplied by environment if cell is to survive and reproduce Classes of growth factors Classes of growth factors amino acids needed for protein synthesis purines and pyrimidines needed for nucleic acid synthesis vitamins function as coenzymes 3 Nutritional classification of Microorganisms Nutritional classification of Microorganisms Nutritional classes : Nutritional classes : Based on carbon source: autotrophs autotrophs use carbon dioxide as their sole or principal carbon source heterotrophs heterotrophs use organic molecules as carbon sources which often also serve as energy source Based on energy source phototrophs phototrophs use light chemotrophs chemotrophs obtain energy from oxidation of chemical compounds Based on electron source lithotrophs lithotrophs use reduced inorganic substances organotrophs organotrophs obtain electrons from organic compounds Nutritional Resource Management Nutritional Resource Management Depending on how carbon, energy and electron sources are used microroganisms can be divided into five nutritional categories: ( auto /hetero) ( photo /chemo) ( litho /organo) Uptake of Nutrients by the Cell Uptake of Nutrients by the Cell...
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Lecture 4-Microbial nutrition colored - 1 Microbial...

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