Biochemistry-lab 3 - Stephanie Darden 2-24-03 Biochemistry...

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Stephanie Darden 2-24-03 Biochemistry Lab #3 Enzyme Specificity – Invertase and Sugars The aim of this experiment was to examine the specificity of invertase by estimating the amount of Carbon dioxide released when various sugars are exposed to the enzyme invertase produced by living yeast cells. My hypothesis is that the sugars will each produce a different amount of Carbon dioxide, and the water will produce no Carbon dioxide. The invertase is specific to certain sugars. Time Control Sucrose Lactose Dextrose Fructose 0 min. 3.9 cm 3.9 cm 3.8 cm 3.9 cm 4.0 cm 20 min. 4.1 cm 5.2 cm 4.0 cm 5.5 cm 5.4 cm 30 min. 3.9 cm 5.8 cm 3.8 cm 6.6 cm 6.4 cm 40 min. 4.0 cm 6.7 cm 4.0 cm 7.4 cm 7.2 cm 50 min. 4.0 cm 7.6 cm 3.9 cm 8.3 cm 8.4 cm 60 min. 4.0 cm 8.9 cm 3.9 cm 9.5 cm 10.0 cm 70 min. 4.0 cm 9.9 cm 4.0 cm 10.1 cm 10.5 cm 80 min. 4.0 cm 10.5 cm 4.0 cm 10.5 cm 11.1 cm 90 min. 4.0 cm 10.8 cm 4.0 cm 10.4 cm 11.8 cm Carbon Dioxide accumulates in the tube where fermentation is occurring. Since
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Biochemistry-lab 3 - Stephanie Darden 2-24-03 Biochemistry...

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