BIOL300%20Lecture%201%20S.H.-1

BIOL300%20Lecture%201%20S.H.-1 - Also used by cancer cells...

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Lecture 1 General principles I Reading for Lecture 1: Lodish Ch. 15, pp. 623-627; Ch. 3, pp. 92-103 Email Siegfried.Hekimi@McGill.ca The answers to the questions of the exam are in the lectures But do read in the textbook pages!!!
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Signal transduction: a signal is converted from one form to another
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Usually at least 7 steps: (1) Synthesis of signaling molecule (2) Release of signaling molecule (ligand) (3) Transport (4) Binding of ligand to receptor (5) Activation of intracellular signal transduction cascade(s) (6) Specific changes in cellular function (7) Termination of response, e.g. by removal of ligand Cell to Cell Signalling
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Every cell employs signal transduction! Yeast mating: diffusible factor (pheromone). shmoo Also bacteria: quorum sensing
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Speed of response: (1) Changes in activity of pre-existing proteins usually fast, “physiological” (2) Changes in the amount of proteins by modification of transcription or RNA processing usually slow, “developmental”
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16_03_signal_various.jpg Different ranges of signaling
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(E) Autocrine signaling allows cells to behave as a group Used by cells in tissue culture;
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Unformatted text preview: Also used by cancer cells to overcome external controls 16_05_target_cells.jpg One ligand can mediate very many different responses And vice versa: different ligands can mediate the same response The active agents in Belladonna, atropine, scopolamine, hyoscyamine have anticholinergic properties. The symptomes include dilated pupils, senstitivity to light, blurred vision, tachycardia, loos of balance, staggering, headache, rash, flushing, dry mouth and throat, slurred speech, urinary retention, constipation, confusion, hallucinations, delirium, convulsions and death. Atropa belladonna; deadly nightshade 16_13_receptor_protein.jpg How do you isolate a ligand biochemically? You need a source: generally a tissue (see text on WebCT) You need an assay Every protein has a unique size Separate proteins according to their properties: every protein has a unique charge Adds negative charge to proteins SDS Cut out bands, renature and test for activity; digest active band with trypsin (protease that cuts after lysine and arginine). Do mass spectrometry...
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BIOL300%20Lecture%201%20S.H.-1 - Also used by cancer cells...

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