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BIOL300%20Lecture%202%20S.H.

BIOL300%20Lecture%202%20S.H. - Lecture 2 General principles...

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Lecture 2 General principles II: Ligand- receptor binding and molecular analysis of signaling proteins Reading for Lecture 2: Lodish Ch. 15, pp. 627-631; Ch. 3, p. 91
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Adds negative charge to proteins SDS
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Cut out bands, renature and test for activity; digest active band with trypsin (protease that cuts after lysine and arginine). Do mass spectrometry
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Mass spectrometry Tryptic peptides are ionized by laser and travel in electric field to detector. Time of flight is inversely proportional to mass. Peptide mass fingerprint can be compared to database of all proteins in an organism
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Purifying the receptor or ligand Ligand-
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