BIOL300%20Lecture%203%20S.H.

BIOL300%20Lecture%203%20S.H. - Lecture 3 General principles...

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Lecture 3 General principles III: Signal transduction cascades (genetic analysis) Reading for Lecture 3: Lodish Ch. 15, pp. 632-634; Ch. 5, pp. 166-167, 209 Ch. 3, p. 90 Ch. 22, 999-1000
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16_08_cascades.jpg altered metabolism, cell shape, or gene expression Production of second messengers Signaling proteins
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Second messengers:
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and other phosphoinositides and calcium
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phosphoserine Eukaryotes have serine/threonine kinases that phosphorylate serines or threonines and tyrosine kinases. Dual-specificity kinases are very rare. About 600 kinases, 100 phosphatases causing phoshorylation of roughly one third of all proteins at any given time Kinase substrates:
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Make a non-phosphorylatable inactive protein: How to analyze the function of kinase substrates
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Make an always “phosphorylated” protein by changing to phosphomimetic amino acid
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Message 1 Message 2 Message 3 Kinases and other relays Response +/- +/- +/- Kinases, or other relays, are integrating device in signaling when they are activated or inhibited by more than one protein.
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This note was uploaded on 10/12/2011 for the course BIOL 300 taught by Professor Nilson during the Spring '11 term at McGill.

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BIOL300%20Lecture%203%20S.H. - Lecture 3 General principles...

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