BIOL300%20Lecture%207%20S.H.

BIOL300%20Lecture%207%20S.H. - Activation of Ras GTPase Sos...

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Activation of Ras GTPase
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Sos is a Ras-GEF! 2 differences between G α and Ras: G α is directly activated by GPCR receptor, G α has higher intrinsic GTPase activity
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The Ras-GEF Sos opens up Ras-GDP so that GDP can diffuse out.
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Raf is kept in an inactive conformation by binding of a 14-3-3 dimer GTP hydrolysis releases active Raf which phosphorylates MEK (ERK) MEK is a dual specificity kinase that phosphorylates one threonine and one tyrosine on the activation lip of MAPK
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Raf is kept in an inactive conformation by binding of a 14-3-3 dimer GTP hydrolysis releases active Raf which phosphorylates MEK (ERK) MEK is a dual specificity kinase that phosphorylates one threonine and one tyrosine on the activation lip of MAPK MAP and ERK Kinase
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anti-diphospho MAPK antibody staining general MAPK antibody would detect staining everywhere Gabay et al. (1997) Development 124, 3535
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Diphospho MAPK activates transcription factors enhancer
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GEF P P
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The Drosophila compound eye can be used to study RTKs genetically
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Each ommatidium is made of 8 photoreceptor R1-R8
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This note was uploaded on 10/12/2011 for the course BIOL 300 taught by Professor Nilson during the Spring '11 term at McGill.

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BIOL300%20Lecture%207%20S.H. - Activation of Ras GTPase Sos...

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