BIOL300%20Lecture%2011%20S.H.

BIOL300%20Lecture%2011%20S.H. - Usually, cells have to...

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Usually, cells have to respond to a combination of signals
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16_39_integrate_signal.jpg Integration of signals/cross-regulation Signal integration/cross-regulation or inhibited Smad1
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Which signal transduction cascade phosphorylates Smad1? EGF increases basal level of Smad1 phosphorylation PI3K inhibitor Phosphorylation is gone in Smad1 mutant and with a MAPKK (MEK) inhibitor
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Which kinase of MAPK cascade phosphorylates Smad1? Activated type I Receptor and [ γ 32 P]ATP causes phosphorylation of wt and mutant Smad1 In vitro kinase assay with recombinant purified proteins: Activated Erk (MAPK) and [ γ 32 P]ATP can only phosphorylate wt Smad1
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What is the consequence of EGF-induced Smad1 phosphorylation? R-Smad/Co-Smad complexes can still form But R-Smad/Co-Smad complex can no longer go to nucleus Smad1 antibody staining
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TGF- β
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16_09_molecules_bind.jpg Intracellular receptors
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16_12_cortisol.jpg Hydrophobic hormones have a slow turnover time, therefore mediating long- term changes.
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16_11_phobic_hormone.jpg Some small and hydrophobic molecules enter cells and bind nuclear receptors Steroid hormones
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Signal Memory (1) Positive feedback by autocrine signaling
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(2) Positive feedback by two signaling systems
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This note was uploaded on 10/12/2011 for the course BIOL 300 taught by Professor Nilson during the Spring '11 term at McGill.

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BIOL300%20Lecture%2011%20S.H. - Usually, cells have to...

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