prokaryote protein synthesis

prokaryote protein synthesis - Protein synthesis is the...

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as E Coli, initiation requires three small proteins called initiation factors, IF1, IF2 and IF3, as well as the first tRNA, the mRNA and the small ribosomal subunit(30S subunit) IF3 readily binds to the small ribosomal subunit, and its presence blocks the large and small subunits from prematurely associating. IF2 facilitates the binding of the mRNA to the small subunit of the ribosome. The binding occurs just four to eight bases upstream of the AUG start, where a consensus sequence, called Shine-Dalgarno sequence, in the mRNA anneals near the end of the 16rRNA in the small ribosomal subunit. IF1 binds to the small subunit at a location called the A site, where incoming tRNAs normally bind. The first tRNA, called N-formylmethionyl-tRNA, thus enters another site, called the P site. Note that the initiator tRNA has been escorted by the P site by IF2, which is bound to GTP, a high-energy molecule similar to ATP. The anticodon of the tRNA is complementary to the AUG start codon.
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This note was uploaded on 10/13/2011 for the course NS 3200 at Cornell.

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prokaryote protein synthesis - Protein synthesis is the...

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