Lecture 9 - protein biosynthesis

Lecture 9 - protein biosynthesis - Mechanistic Aspects of...

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Unformatted text preview: Mechanistic Aspects of ribosome-mediated translation. The message contained in mRNA is translated into protein from aminoacyl-tRNA. This process can be divided into three phases: chain initiation, elongation and termination. Components: i. Initiation, elongation and release factors ii. ribosome iii. met-tRNA (met) and aminoacyl-tRNAs iv. mRNA v. nucleoside triphosphate (ATP and GTP) Translation initiation: Chain initiation: Three general steps are required. The first two involve the binding of 40S subunit to initiator tRNA and mRNA at AUG. In the third step the 60S subunit join this complex, and the initiation factors dissociate. For Procaryotes - The SD sequence is required for initiation. For Eucaryotes Cap binding protein binds to 5' end of mRNA (Cap increases translational efficiency). Additional proteins bind, then 40 S ribosomal subunit binds. The complex moves to first ATG (requires ATP hydrolysis). Additional proteins bind, then 60S subunit binds. Translation proceeds till a stop codon is reached (UGA;UAA;UAG). Ribosome has three "sites: E site - exit site P site - peptidyl site A site - acceptor site Elongation of peptide chain:...
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Lecture 9 - protein biosynthesis - Mechanistic Aspects of...

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