Lecture 10 - protein processing

Lecture 10 - protein processing - Inhibitors of Protein...

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Inhibitors of Protein Synthesis Protein synthesis can be affected by many factors: nutritional, physiological, as well as pathological conditions. Many antibiotics function by inhibiting ribosome-mediated translation of mRNA into polypeptide. Some antibiotics are inhibitors of protein synthesis in both eukaryotes and prokaryotes, others are specific inhibitors only to either eukaryotes or prokaryotes. If the inhibitor is sufficiently specific to prokaryotes it will be useful clinically.
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Puromycin is a structure analog of the terminal aminoacyl-adenosine portion of aminoacyl-- tRNA.
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Mode of action : Puromycin binds to "A" site on the ribosome and inhibits the entry of aminoacyl-tRNA. The amino group can form a peptide bond with peptidyl tRNA on the "P" site resulting peptidyl puromycin which is subsequently released. Thus, puromycin causes premature chain termination. Protein synthetic systems : Puromycin inhibits protein synthesis in both procaryotes and eucaryotes.
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Cycloheximide 1. Structure: Cycloheximide is a glutarimide isolated from streptomyces griseus . 2. Mode of action: Cycloheximide binds to 60S subunit of the 80S ribosome inhibiting the "peptidyl transferase" activity . 3. Protein synthetic systems: Cycloheximide inhibits only protein synthesis in eukaryotes.
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Clinically useful antibiotics: Aminoglycosides (streptomycin, spectinomycin, neomycin, kanamycin), tetracyclines, and erythromycin are highly effective antibacterial agents. They interfere with prokaryotic protein synthesis at various phases of ribosomes mediated translation. Streptomycin and related aminoglycosides:
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This note was uploaded on 10/13/2011 for the course NS 3200 at Cornell.

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Lecture 10 - protein processing - Inhibitors of Protein...

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