LIPIDS lab report draftW11

LIPIDS lab report draftW11 - BIOLOGY 1510 Lab Section 000 I...

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BIOLOGY 1510 Lab Section 000 Ima Goodstudent LIPIDS LAB REPORT DRAFT INTRODUCTION Three experiments showing characteristics of a lipid Paper test used a drop of oil on plain paper The test tube test mixed oil and water Add soap to show how it breaks down the oil causes it to mix with the water TLC separates the glycerol from the 3 fatty acids Lipids broken into component parts by hydrolysis, reverse process is dehydration synthesis (Van Thiel 3) Lipid structure Glycerol and 3 fatty acids (COOH group and R group) Can be unmixed lipid or mixed lipid (Van Thiel 3) unmixed lipid (all 3 fatty acids the same) mixed lipid (2 or 3 different fatty acids) Differences between saturated, unsaturated, and polyunsaturated (Wikipedia) hydrogenation and number of H’s attached number of double bonds solid vs. liquid Chromatography separates and identifies different biomolecules including lipids (Van Thiel 4) The TLC paper is stationary phase Chromatography solvent is mobile phase Used ascending chromatography as the solvent moved up the paper via capillary action MATERIALS AND METHODS Procedures for experiment per page 6 of Experiments in Biology From Chemistry to Sex (Van Thiel 6)
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This note was uploaded on 10/13/2011 for the course BIO 1510 taught by Professor Rodriguez during the Fall '08 term at Wayne State University.

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LIPIDS lab report draftW11 - BIOLOGY 1510 Lab Section 000 I...

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