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PV 92 Questions Students - 5 What are the three main steps...

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PV 92 Questions Lesson 1 DNA Template Preparation 1. Why is it necessary to chelate the metal ions out of solution during the boiling/lysis step at 100°C? 2. What would happen if you did not put in the InstaGene™ matrix? 2. What is needed from the cells to conduct the polymerase chain reaction (PCR)? 3. What structures must be broken to release the DNA from a cell? Lesson 2 PCR Amplification 1. Why is it necessary to have a primer on each side of the DNA sequence to be amplified? 2. How did TaqDNA polymerase acquire its name? 3. Why is TaqDNA polymerase important tool for pcr? 4. Why are there nucleotide bases (A, T, G, and C) in the master mix?
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Unformatted text preview: 5. What are the three main steps of PCR amplification? 6. Choose one of the three main steps of each cycle of PCR amplification and describe what reactions occur. Lesson 3 Gel Electrophoresis 1. What is an intron? 2. What is an exon? 3. Explain how agarose electrophoresis separates DNA fragments of interest. 4. Why does a smaller DNA fragment move faster than a larger one? 5. What kind of controls are run in this experiment? 6. Why are experimental controls important, especially in this situation? 7. Be able to identify the genotypes for the Alu insert in your PV92 region if given a drawing, photograph or sample gel....
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