Unformatted text preview: 5. What are the three main steps of PCR amplification? 6. Choose one of the three main steps of each cycle of PCR amplification and describe what reactions occur. Lesson 3 Gel Electrophoresis 1. What is an intron? 2. What is an exon? 3. Explain how agarose electrophoresis separates DNA fragments of interest. 4. Why does a smaller DNA fragment move faster than a larger one? 5. What kind of controls are run in this experiment? 6. Why are experimental controls important, especially in this situation? 7. Be able to identify the genotypes for the Alu insert in your PV92 region if given a drawing, photograph or sample gel....
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- Fall '08
- DNA, DNA sequencing, pcr amplification