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BE115+-+Lab09 - Lab 9 DNA Electrophoresis 1 Introduction...

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Page 1 of 4 Bioengineering 115: Cell Biology for Engineers Laboratory 9: DNA Electrophoresis Last updated: January 21, 2011 Lab 9: DNA Electrophoresis 1 Introduction You have already poured and run vertical gels for separation of proteins. During this lab, you will work in groups of four to pour and run horizontal gels for size separation of nucleic acids. You will then prepare and load samples of you RT-PCR products from Lab 8. 2 Objectives To perform DNA electrophoresis in a horizontal agarose gel To evaluate RT-PCR products from Lab 8 experiments 3 References Gel electrophoresis (http://www.dnalc.org/resources/animations/gelelectrophoresis.html) 4 Reagents, Supplies, and Equipment 4.1 Reagents 1. 6X DNA Sample buffer (30% glycerol in dH 2 0) 2. UltraPure™ 10X TBE Buffer (Invitrogen 15581-044) 3. SYBR Safe in 0.5X TBE Buffer (Invitrogen s33110) 4. Agarose (Super Resolution) – (Genessee 20-106) 5. 100 bp DNA Ladder – (Invitrogen 15628019) 4.2 Supplies 1. Plastic Wrap 4.3 Equipment 1. Horizontal electrophoresis unit 2. Power Source 3. Safe Imager (www.invitrogen.com/safeimager)
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Page 2 of 4 Bioengineering 115: Cell Biology for Engineers Laboratory 9: DNA Electrophoresis Last updated: January 21, 2011 5 Protocol Each gel will be poured, loaded, and run by groups of four.
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