BE115+-+Lecture02 - Lecture 2 2D and 3D Cell Culture...

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Lecture 2 2D and 3D Cell Culture "The best model of a cat is another cat or, better, the cat itself." - Norbert Wiener 1 BE115 Spring 2011
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Homework - Image Analysis 2 BE115 Spring 2011
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Homework - using Image J 3 Threshold: 150 Eraser work: minimal Number of cells: 21 Cell area: 42 +/- 10 µm2 Angle: 82 +/- 23 degrees BE115 Spring 2011
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Homework - Sensitivity Analysis 4 Cell Number: ~1% change in cell number/unit threshold Area: ~1% change in area/unit threshold Angle: negligible change in angle/unit threshold Questions: Are these sensitivities acceptable for your planned experiment? How would you decide? BE115 Spring 2011
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Image Analysis Counting cells via the computer is quick and easy Developing a protocol to teach the computer how to count cells is neither quick nor easy Different outputs are more or less sensitive to changes in your settings When using Image J, be aware of these sensitivities! 5 BE115 Spring 2011
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Question: “You want to determine the effect of drug X on the instructor. What are the possible models you could test drug X on? 6 BE115 Spring 2011
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Models The instructor Other humans Animal models Some animals are better models than others Cell culture models Hundreds of different kinds of cells to test Thousands of different culture conditions Virtual models (improving every day) 7 Representative E a s y t o u e BE115 Spring 2011
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A Sample Progression 8 BE115 Spring 2011
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Cell Types 9 BE115 Spring 2011
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Primary Cells Origin: Isolated from animal tissue Representative? Highly representative – they act like cells from the tissue they’re taken from Homogeneity: Disturbingly individual – the age, sex, and treatment of a particular animal all have an effect Maintenance: Difficult – individual cells used to a tissue ( in vivo ) environment tend to lose normal function over time (or die) Lifespan: Short – from hours to a few weeks at most 10 BE115 Spring 2011
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Finite Cell Lines Origin: Usually from fetal animal tissue Representative? Highly representative
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BE115+-+Lecture02 - Lecture 2 2D and 3D Cell Culture...

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