BE115+-+Lecture04 - Lecture 4 Analysis of 3D Cell Culture...

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Lecture 4 Analysis of 3D Cell Culture "A mouse is an animal that, if killed in sufficiently many and creative ways, will generate a PhD."
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Tissue Analysis How is investigating tissue SIMILAR to investigating cells? How is investigating tissue
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Experimental outputs for tissue Tissue visualization ECM characterization Composition Histology Mechanical testing Etc.
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Histology Sectioning of tissue into thin (~10 micron) slices Often followed by chemical or antibody stains are used to highlight different features of the tissue section Unstained sections can be subjected to birefringence or FTIR
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Histology of Liver
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3D Histology 3D reconstructions of the original tissue can be created by computer image analysis In these cases, often the slicing is automated to aid in reconstruction Individual Sections of Rabbit Brain Reconstruction MRI http://www.itg.uiuc.edu/publications/forums/1999-05-27/
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Micro-CT Virtual Histology Microcomputed tomography X-ray cross- sections integrated into a 3D reconstruction Mouse Embryo
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Tissue Development Four-dimensional analysis of vascularization during primary development of an organ, the gonad
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ECM Composition Integrins on the cell surface interact with and attach to ECM There are various integrin types, each recognizing different ECM molecules Composition of the ECM can be determined via Western blot
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Vessel Tendon Ligament Bone Cartilage Cells ~10% 25-75% 1-5% 15-25% 5-10% ECM ~90% 25-75% 95-99% 75-85% 90-95% Exact amount varies across location, age, and species. We are not wall-to-wall cells!
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This note was uploaded on 10/14/2011 for the course BIOE 115 taught by Professor Haley during the Spring '11 term at City College of San Francisco.

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BE115+-+Lecture04 - Lecture 4 Analysis of 3D Cell Culture...

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