BE115+-+Lecture05 - BE115 Lecture 6 1 Projects You should...

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BE115 Lecture 6 1
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Projects You should start thinking about an interesting question for you and your group to work on for the final project If you haven’t posted a paper on the blog yet, this is a great way to find a good topic Groups finalized with a general goal by Week 7 Concrete goals to be finalized by Week 9 2
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Fluorescence Microscopy Fluorescent dyes or fluorescently-labeled antibodies Three major colors Fluorescein (FITC) excited by blue, emits green Rhodamine excited by green-yellow, emits red DAPI excited by violet, emits blue FITC, Rhodamine, and DAPI are the “classic” fluorescent dyes of each color - there are many other red, green, and blue fluorescent dyes 3
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Fluorescent Filters A set of excitation and emission filters are used to bring out a particular color Various fluorescent molecules excite and emit at different wavelengths Multicolor images are achieved by adding 4 Sample Eyepiece
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Protocol for Quantifying Fluorescence What factors would influence comparisons of fluorescence intensity of different cells… …on the same image? …on the same slide? …on different slides? …on slides from a previous experiment? How would you account for possible autofluorescence? What are the consequences of saturated pixels in a bright image? 5
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Lecture 5 Protein Quantification "To consult a statistician after an experiment is finished is often merely to ask him to conduct a post-mortem examination. He can perhaps say what the experiment died of." - R.A.Fisher 6
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Proteins A human body is ~20% protein by weight
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This note was uploaded on 10/14/2011 for the course BIOE 115 taught by Professor Haley during the Spring '11 term at City College of San Francisco.

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BE115+-+Lecture05 - BE115 Lecture 6 1 Projects You should...

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