BE115+-+Lecture10 - Lecture 10 Project Proposals &...

Info iconThis preview shows pages 1–6. Sign up to view the full content.

View Full Document Right Arrow Icon
Lecture 10 Experimental Design 1
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Presentation Order Reprogramming of 3T3 Cells using Electroporation Team Awesome Team EGF Josh’s CurD and Jam Oy Gevalt RNAces IncrediCells Team RANT 2
Background image of page 2
Reprogramming of 3T3 Cells using Electroporation of Minicircle Vector Daniel Chen, Angela Shi, Chia-Hung Tai, Charlie Yeh Background/Motivation o Differentiated cells can be reprogrammed into iPS cells by introducing certain transcription factors (OCT4, NANOG, SOX2, LIN28) o Current research on method to increase success rate and prevent mutagenesis (caused by viral vectors) o Wu et al (2010) reprogrammed adult adipose stem cells using a minicircle vector containing all 4 transcription factors using electroporation Our ideas o Is it possible to reprogram common cancer cell lines such as 3T3 fibroblasts using electroporation? o Can we improve the efficiency of this method by using on-chip electroporation?
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Our Plan Establish off-chip electroporation protocol with a simple molecule (Propidium Iodide) Off-chip electroporation with minicircle vector On-chip electroporation with Propidium Iodide On-chip electroporation with minicircle vector If useful results can be collected
Background image of page 4
Project Goal HER-2 protein over-expressed in breast cancer Transmembrane protein To ensure lysate from microfluidic device contains ample protein in a stable and detectable form To develop a coupled immunoassay with the device for rapid testing of breast cancer Mansi Shah Daniel Yang Eric Johnson Hinesh Patel Novel PDMS microfluidic device for cell trap and release Electrochemical lysis within specific region of device Team Members The Device Specific Protein Motivation To characterize the difference between on-chip and off-chip lysis of BT-474 breast cancer cells.
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 6
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 10/14/2011 for the course BIOE 115 taught by Professor Haley during the Spring '11 term at City College of San Francisco.

Page1 / 24

BE115+-+Lecture10 - Lecture 10 Project Proposals &...

This preview shows document pages 1 - 6. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online