Lecture01 - Lecture 1 Introduction Tissue Culture “The...

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Unformatted text preview: Lecture 1 Introduction, Tissue Culture “The most exciting phrase to hear in science, the one that heralds new discoveries, is not 'Eureka!', but 'That's funny...‘”- Isaac Asimov “That's the nature of research - you don't know what in hell you're doing.”- 'Doc' Edgerton 1 BE115 Lecture 1 Fall 2010 BE 115: Cell Biology Laboratory for Engineers Instructor: Hayley Lam & Terry Johnson Units: 4 Lecture: Mondays, 5-7 PM Labs: Thursdays or Fridays, 2-6PM 2 BE115 Lecture 1 Fall 2010 Show of hands Who wants to live forever? 3 BE115 Lecture 1 Fall 2010 Aurora, goddess of the morning and Tithonus, Prince of Troy 4 • Aurora asked Zeus to make Tithonus immortal , but never mentioned unaging • The relationship ended poorly . Baby, it’s not me…it’s you. BE115 Lecture 1 Fall 2010 Tissue Engineering • Problem: tissues are damaged by trauma, disease or age • Current solutions: – Transplants – Artificial implants • Preferred solution: – Tissue Engineering: The manipulation of cells and tissue into behavior (growth, differentiation, migration, etc.) that allows us to build either new tissue or biocompatible scaffolds 5 BE115 Lecture 1 Fall 2010 Show of hands How many of you are planning to go to… Graduate school? Medical school? Industry? 6 BE115 Lecture 1 Fall 2010 Class Goals • Build a Bioengineering Toolbox: – familiarize you with molecular biology lab techniques that every bioengineer should know – Understand the advantages and limitations of each – Three bullet points for your resume: • Mammalian tissue culture • SDS-PAGE/Western blotting • RT-PCR • Build a Bioengineering Thinking Cap: – How do you design experiments to test a question? – Treatment and presentation of data 7 BE115 Lecture 1 Fall 2010 Grading Policy • Weekly Quizzes – 40% • Lab Notebook/Homework – 10% • Class participation – 10% • Project - 40% • Proposal and experimental plan – 5% • Oral presentation – 5% • Paper – 30% 8 BE115 Lecture 1 Fall 2010 Weekly Quizzes • 20-25 minutes, beginning of lecture • Will tend to focus on: – Last week’s lecture – Last week’s lab – Next lab – Homework and any reading assignments 9 BE115 Lecture 1 Fall 2010 Class Participation • Be prepared to… – Actively participate in class discussions and activities – Share your homework and lab results – Discuss any readings or other assignments – Learn in different ways Fall 2010 BE115 Lecture 1 10 Class Project • Putting your new toolbox to use: an exercise in experimental design, implementation, and dissemination • Important dates: • Week of March 7th – Form project groups and brainstorm project ideas • March 14th – Project concept and contract due Fall 2010 BE115 Lecture 1 11 Lab Notebooks • There is a “homework” at the end of each lab protocol • You are responsible for having your notebook checked by your GSI before leaving the lab • We will be looking for: – Last week’s lab: results, conclusions, homework – This week’s lab: prelab (sections 1-4) and 12 BE115 Lecture 1 Fall 2010 Choosing a notebook...
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This note was uploaded on 10/14/2011 for the course BIOE 115 taught by Professor Haley during the Spring '11 term at City College of San Francisco.

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Lecture01 - Lecture 1 Introduction Tissue Culture “The...

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