Lecture01 - Lecture 1 Introduction, Tissue Culture The most...

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Unformatted text preview: Lecture 1 Introduction, Tissue Culture The most exciting phrase to hear in science, the one that heralds new discoveries, is not 'Eureka!', but 'That's funny...- Isaac Asimov That's the nature of research - you don't know what in hell you're doing.- 'Doc' Edgerton 1 BE115 Lecture 1 Fall 2010 BE 115: Cell Biology Laboratory for Engineers Instructor: Hayley Lam & Terry Johnson Units: 4 Lecture: Mondays, 5-7 PM Labs: Thursdays or Fridays, 2-6PM 2 BE115 Lecture 1 Fall 2010 Show of hands Who wants to live forever? 3 BE115 Lecture 1 Fall 2010 Aurora, goddess of the morning and Tithonus, Prince of Troy 4 Aurora asked Zeus to make Tithonus immortal , but never mentioned unaging The relationship ended poorly . Baby, its not meits you. BE115 Lecture 1 Fall 2010 Tissue Engineering Problem: tissues are damaged by trauma, disease or age Current solutions: Transplants Artificial implants Preferred solution: Tissue Engineering: The manipulation of cells and tissue into behavior (growth, differentiation, migration, etc.) that allows us to build either new tissue or biocompatible scaffolds 5 BE115 Lecture 1 Fall 2010 Show of hands How many of you are planning to go to Graduate school? Medical school? Industry? 6 BE115 Lecture 1 Fall 2010 Class Goals Build a Bioengineering Toolbox: familiarize you with molecular biology lab techniques that every bioengineer should know Understand the advantages and limitations of each Three bullet points for your resume: Mammalian tissue culture SDS-PAGE/Western blotting RT-PCR Build a Bioengineering Thinking Cap: How do you design experiments to test a question? Treatment and presentation of data 7 BE115 Lecture 1 Fall 2010 Grading Policy Weekly Quizzes 40% Lab Notebook/Homework 10% Class participation 10% Project - 40% Proposal and experimental plan 5% Oral presentation 5% Paper 30% 8 BE115 Lecture 1 Fall 2010 Weekly Quizzes 20-25 minutes, beginning of lecture Will tend to focus on: Last weeks lecture Last weeks lab Next lab Homework and any reading assignments 9 BE115 Lecture 1 Fall 2010 Class Participation Be prepared to Actively participate in class discussions and activities Share your homework and lab results Discuss any readings or other assignments Learn in different ways Fall 2010 BE115 Lecture 1 10 Class Project Putting your new toolbox to use: an exercise in experimental design, implementation, and dissemination Important dates: Week of March 7th Form project groups and brainstorm project ideas March 14th Project concept and contract due Fall 2010 BE115 Lecture 1 11 Lab Notebooks There is a homework at the end of each lab protocol You are responsible for having your notebook checked by your GSI before leaving the lab We will be looking for: Last weeks lab: results, conclusions, homework This weeks lab: prelab (sections 1-4) and 12 BE115 Lecture 1 Fall 2010 Choosing a notebook...
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Lecture01 - Lecture 1 Introduction, Tissue Culture The most...

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