{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

Gluconeogenesis practice problem key

Gluconeogenesis practice problem key - BMB...

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: BMB 401Gluconeogenesis Practice problems Page 1 1) Please state the three glycolytic enzymes that catalyze irreversible reactions. and indicate the gluconeogenic enzyme or enzymes that are used to go around each roadblock. a. Glycogenic enzyme__Hexokinase_________________ b. Gluconeogenic enzyme(s)___Glucose 6 ­phosphatase_______ ____________________________________________________________________________________ a. Glycogenic enzyme__Phosphofructokinase ____________________________ b. Gluconeogenic enzyme(s)____ Fructose 1,6 ­bisphosphatase _______ ____________________________________________________________________________________ a. Glycogenic enzyme_________Pyruvate kinase__________________________________ b. Gluconeogenic enzyme(s)__ o Pyruvate carboxylase, (carboxylates pyruvate to make OAA) __________ o Malate Dehydrogenase (The mitochondrial isozyme reduces OAA to form malate and then the cytoplasmic isozyme catalyzes the reverse reaction) o Phosphoenolpyruvate carboxykinase (decarboxylates and phosphorylates OAA to form phosphoenolpyruvate) 2) During gluconeogenesis oxaloacetate must be changed to malate in what intracellular compartment? (cytosol/mitochondrial matrix) 3) Why must oxaloacetate be changed to malate in this compartment?___________ _There is no transporter for OAA in the inner mitochondrial membrane to transport OAA from one compartment to the other___________________________ 4) How is this pathway regulated? Please state each enzyme and the metabolite that increases or decreases the activity of each enzyme__________________________ o G6Phosphatase: Information on the regulation of this enzyme is not required in this class. o Fructose 1,6 ­bisphosphatase: Positive: Citrate, Negative: F2, 6 ­BP and AMP o Phosphoenolpyruvate carboxykinase: Negative: ADP o Pyruvate carboxylase: Positive: Acetyl CoA, Negative: ADP 5) Please state the meaning of “reciprocal regulation” This means that the same signal that inhibits one pathway will activate the opposite pathway. For instance F2, 6 ­BP deactivates gluconeogenesis and activates glycolysis. This reduces the occurrence of futile cycles of concurrent anabolism & catabolism. ...
View Full Document

{[ snackBarMessage ]}

Ask a homework question - tutors are online