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Glycolysis practice problems KEY

Glycolysis practice problems KEY - Practice problems BMB401...

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Unformatted text preview: Practice problems BMB401: Glycolysis Page 1 1) Please write out the reaction that is catalyzed by PFK1 (phosphofructokinase 1). (you do not need to draw out the structures) Fructose 6 ­P + ATP  Fructose 1,6 ­BP + ADP 2) Please state the difference between a substrate ­level phosphorylation and phosphorylation by kinase reaction. a) During substrate level phosphorylation, a phosphate group is transferred from a reactant with higher phosphoryl transfer potential to a substrate to make a product with a lower phosphoryl transfer potential. Please see table 15.1 – you should know which 3 metabolic intermediates have a higher phosphoryl transfer potential than ATP. b) Kinase reactions transfer a phosphate group from ATP to a substrate to make a phosphorylated product with a lower phosphoryl transfer potential than that associated with ATP. 3) Please name the two glycolytic enzymes that participate is kinase ­powered phosphorylation: a. _Hexokinase b. _Phosphofructokinase 1 4) Please name the two glycolytic enzymes that participate in substrate ­level phosphorylation. a. Phosphoglycerate kinase (named for the reverse reaction) b. Pyruvate Kinase 5) Please name the enzyme that catalyzes the reaction shown below: a. _Pyruvate Kinase __________________________________________________________________ Practice problems BMB401: Glycolysis Page 2 6) Please state the major fate of pyruvate in hypoxic conditions. (Note: Hypo means insufficient, so hypoxic means insufficient oxygen) Please include the reactants, products and enzyme catalyst Major fate is lactate, as pyruvate is reduced to provide NAD+ for glycolysis to continue Pyruvate + NADH yield Lactate + NAD+ + H+. 7) Please state the step in the glycolytic pathway at which the metabolic intermediates of fructose catabolism can enter. a. In the liver: Glyceraldehyde (which must be phosphorylated to glyceraldehyde 3 ­phosphate) and dihydroxyacetone ­phosphate b. In adipose tissue: Fructose 6 ­phosphate 8) Because of this entry point, what is the first point of regulation that regulates flow of these metabolites of fructose through glycolysis? a. In the liver: Pyruvate Kinase b. In adipose tissue: PFK1 9) Please state the reaction catalyzed by the enzyme: galactose 1 ­phosphate uridyltransferase, (include all reactants and products of this reaction). Galactose 1 ­P + Glucose ­UDP yield Galactose ­UDP + Glucose 1 ­Phosphate 10) Lactose is a disaccharide comprised of galactose and glucose. ...
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