Practice Exam 2 - Practice exam BMB 401 Exam 2 ...

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Unformatted text preview: Practice exam BMB 401 Exam 2 Page 1 1) a ­c. Please state three of the major products of the glycolytic pathway a_______________________________ b______________________________ c_____________________ d) Please identify the enzyme that catalyzes the first committed step of glycolysis.____________________________________ 2) a ­b. Two enzymes were discussed regarding catalysis of the first reaction of glycolysis. Please name these two enzymes that can catalyze this reaction. a__________________________________________ b___________________________________________ c. Which of these two enzymes is negatively regulated by its product? _____________________ d. What is this product?? ___________________________________________________ 3) a. During glycolysis, a 6 ­carbon molecule is split in to two 3 ­carbon molecules. What is the enzyme that catalyzes this reaction? ____________________ ______________________________________________________________________________________ b. ­c. Please name the two 3 ­carbon molecules which are the products of this reaction. b___________________________________________c___________________________________________ d. Does the enzyme, listed in part 3a, catalyze the reverse reaction during gluconeogenesis?____________________________________________________ 4) Please name two cofactors that act as carriers of carbon containing groups a ______________________________________b ___________________________ 5) Name two vitamins that do not function as cofactors. a)________________________________________________b_____________________________________ 6) a)What global signal stimulates the rate of glycolysis?_________________________________ b)This global signal is released when serum glucose levels are {high, low}. 7) Please state two means by which the enzyme, δ ­aminolevulinate synthase (δALA), is regulated. a)____________________________________________________________________________________ b)____________________________________________________________________________________ 8) During the biosynthesis of heme, the symmetric ring, uroporphyrinogen III is formed. TRUE FALSE 9) a) Please state the biochemical basis for acute intermittent porphyria.__________________________________________________________________________________________ ___________________________________________________________________________ b) Please name the major transporter of iron in the blood. __________________________________ 10) Please state the organ location and the purpose of biliverdin reductase a)Location:_____________________________________________________________________ b) Purpose______________________________________________________________________ 11) Bilirubin is transported to the liver by ___________________________________________ 12) ∆G‡ is defined as:___________________________________________________________________________ ________________________________________________________________________________________ Practice exam BMB 401 Exam 2 Page 2 13) a) Tightly bound, inorganic cofactors: {Metals, Prosthetic groups, Coenzymes} b) Small, Organic Molecules that act as loosely bound cofactors: {Metals, Prosthetic groups, Coenzymes} 14) One hallmark of non ­competitive inhibition is that Km {>, <, =} Kmapp 15) Please identify the vitamin in Figure 1_______________________ 16) a) Please state the purpose of Coenzyme A as a cofactor: ______ ___________________________________________________________________________ ___________________________________________________________________________ b) From which vitamin is this cofactor derived?________________ Figure 1 ______________________________________________________________________________________ 17) a Please name 4 of the lipid soluble vitamins: ____________________________________ 19b Please state the basis for the condition: beri beri. _________________________________________ ______________________________________________________________________________________________________ 18) a) What is the function of E1 in the pyruvate dehydrogenase complex?___________________________________________________________________________________________ ____________________________________________________________________________________ b) What cofactor is used by E1?_________________________________________________________ c) From what vitamin is this cofactor derived?________________________________________ d) What does this cofactor do specifically?____________________________________________ 19) Please name 2 enzymes that catalyze irreversible steps in the glycolytic pathway a)______________________________________________b)__________________________________________ 20) Please name 2 enzymes in the gluconeogenic pathway that are used to go around the “roadblock” of glycolytic reaction 10. a_______________________________________________ b)_________________________________________ 21) Please describe the major function of fructose 2,6 ­bisphosphate__________________ _____________________________________________________________________________________________ 22) At what point or points does fructose join the glycolytic pathway? ________________ ____________________________________________________________________________________________ _____________________________________________________________________________________________ Practice exam BMB 401 Exam 2 Page 3 23) By using Graph 1, Where Vo is in µM/sec, and S is in µM, please solve for: a)Kmapp_______________________________ b) Vmax______________________________ c) What type of graph is this?____________________________________ ______________________________________________ d) What type of inhibition is seen?___________________________________ 24) a) Please use Figure 2 below to answer the following questions: During glycolysis, is a is converted to b, or is b converted to a? ______________________________________________ ______________________________________________ b) Is this a reversible or irreversible reaction?_____________________________________ c) What is the purpose of this reaction? ____________________________________________ ________________________________________________ ________________________________________________ ________________________________________________ Figure 2 ______________________________________________________________________________________________________ ____________________________________________________________ d) Please identify the intermediate shown in Figure 2b_______________________________________ 25) Please name two positive effectors and two negative effectors of the enzyme that catalyzes the reaction discussed in question 24 a) Positive __________________________________________________________________________ b) Positive __________________________________________________________________________ c) Negative __________________________________________________________________________ d) Negative __________________________________________________________________________ 26) EC1) What enzyme catalyzes the reaction discussed in questions 24 and 25? ______________________a ­c. Please state three of the major products of the glycolytic pathway a_______________________________ b______________________________ c_____________________ d) Please identify the enzyme that catalyzes the first committed step of glycolysis.____________________________________ Practice exam BMB 401 Exam 2 Page 4 27) a ­b. Two enzymes were discussed regarding catalysis of the first reaction of glycolysis. Please name these two enzymes that can catalyze this reaction. a__________________________________________ b___________________________________________ c. Which of these two enzymes is regulated? ______________________________________ d.How is this enzyme regulated? ___________________________________________________ _________________________________________________________________________________________ 28) a. During glycolysis, a 6 ­carbon molecule is split in to two 3 ­carbon molecules. What is the enzyme that catalyzes this reaction? ____________________ ______________________________________________________________________________________ b. ­c. Please name the two 3 ­carbon molecules which are the products of this reaction. b___________________________________________c___________________________________________ d. Does the enzyme, listed in part 3a, catalyze the reverse reaction during gluconeogenesis?____________________________________________________ 29) Please name two cofactors that act as carriers of carbon containing groups a ______________________________________b ___________________________ 30) Name two vitamins that do not function as cofactors. a)________________________________________________b_____________________________________ 31) What global signal stimulates the rate of glycolysis?_________________________________ 32) Please state two means by which the enzyme, δ ­aminolevulinate synthase (δALA), is regulated. a)____________________________________________________________________________________ b)____________________________________________________________________________________ 33) During the biosynthesis of heme, the symmetric ring, uroporphyrinogen III is formed. TRUE FALSE 34) Please state the biochemical basis for acute intermittent porphyria.__________________________________________________________________________________________ ___________________________________________________________________________ 35) Please name the major transporter of iron in biologic systems. ______________________________________________ 36) Please state the organ location and the purpose of bilirubin reductase a)Location:_____________________________________________________________________ b) Purpose______________________________________________________________________ 37) Bilirubin is transported to the liver by ___________________________________________ 38) ∆G‡ is defined as:___________________________________________________________________________ ________________________________________________________________________________________ 39) Tightly bound, inorganic cofactors: {Metals, Prosthetic groups, Coenzymes} 40) Small, Organic Molecules that act as loosely bound cofactors: {Metals, Prosthetic groups, Coenzymes} Practice exam BMB 401 Exam 2 Page 5 41) One hallmark of non ­competitive inhibition is that Km {>, <, =} Kmapp 42) a Please identify the vitamin in Figure 1_______________________ 43) a) Please state the purpose of Coenzyme A as a cofactor: ?_______ ___________________________________________________________________________ ___________________________________________________________________________ b) What vitamin is this cofactor derived from?________________ Figure 1 ______________________________________________________________________________________ 44) Please name 4 of the lipid soluble vitamins: ?____________________________________ 45) Please state the basis for the condition: beri beri. ____________________________________________ ______________________________________________________________________________________________________ 46) a) What is the function of E1 in the pyruvate dehydrogenase complex?___________________________________________________________________________________________ ____________________________________________________________________________________ b) What cofactor is used by E1?_________________________________________________________ c) From what vitamin is this cofactor derived?________________________________________ d) What does this cofactor do specifically?____________________________________________ 47) Please name 2 enzymes that catalyze irreversible steps in the glycolytic pathway a)______________________________________________b)__________________________________________ 48) Please name 2 enzymes in the gluconeogenic pathway that are used to go around the “roadblock” of glycolytic reaction 10. a_______________________________________________ b)_________________________________________ 49) Please describe the major function of fructose 2,6 ­bisphosphate__________________ _____________________________________________________________________________________________ 50) At what level, what step 1 ­10, does fructose join the glycolytic pathway?________________ EC) What is the first regulation that effects utilization of fructose in the glycolytic pathway? ______________________________________________________________________________________________________________ ______________________________________________________________________________________________________________ EC2) N-linked complex carbohydrates are transferred en bloc to asparagine residues on proteins in the endoplasmic reticulum. True False ...
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