Pyruvate Dehydrogenase Complex Practice key

Pyruvate Dehydrogenase Complex Practice key - BMB 401...

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Unformatted text preview: BMB 401 Pyruvate Dehydrogenase Complex Page 1 Practice Problems 1) Please name the 5 cofactors that participate in the function of PDC, and state the function of each. a. * Thiamine Pyrophosphate (TPP) is part of E1 This cofactor, when in its anionic form assists the decarboxylation of pyruvate by nucleophilic attack of the pyruvate carbonyl group. b. * Lipoamide is part of E2 This cofactor has a long swing arm and transfers the acyl group from hydroxyethylTPP to Coenzyme A c. * FAD is part of E3 This cofactor helps to reconstitute the functioning complex. It oxidizes the reduced lipoamide (dihydrolipoamide),to form the disulfide bridge. d. * NAD+ is a coenzyme (Unlike FAD cofactors, which associate strongly with the enzymes that they function in, NAD+, comes and goes freely and is not tightly associated with any one enzyme) NAD+ oxidizes reduced FADH2 to produce the FAD2 needed for E3 function. e. CoEnzyme A. This also is a coenzyme and does not associate with any one enzyme tightly. This coenzyme carries and activates the acetyl group. 2) Please write out the reaction catalyzed by the PDC. Pyruvate + CoA + NAD+  ­> Acetyl CoA + CO2 + NADH + H+ 3) The pyruvate hydrogenase complex is located in the (cytosol / outer mitochondrial membrane/ mitochondrial matrix). 4) The PDC is similar to which of the following: (alpha ­ketoglutarate dehydrogenase complex / phosphofructokinase 1) 5) The starting glycolytic metabolite has how many carbons? _3______. The ending acyl group has how many carbons?_2________. ...
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This note was uploaded on 10/18/2011 for the course BMB 401 taught by Professor Kaguni during the Fall '08 term at Michigan State University.

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