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ch6thecell - Ch6 A tour of the cell 1 The cell is smaller...

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Ch6 A tour of the cell. 1. The cell is smaller than the resolution of the human eye <0.1mm. So humans cannot see these items. The study of the cell developed with the discovery/invention of tools to look at them. a. Microscopy: Looking at the cell i. Looking at small things 1. magnification 2. resolution 3. contrast 4. Thin sections ii. Light microscope. Resolution to very small prokaryotic cells 1. bright field 2. phase contrast iii. Electron microscope. Resolution down to large macromolecular structures 1. transmission 2. Scanning b. Cell fractionation: Is a way to correlate the structures seen in microscopy with what is really going on. i. Disruption of the cell ii. Centrifugation of portions of the cell based on size iii. Chromatography separation of cell components based on affinity to a substrate 2. The Eukaryotic/ prokaryotic split a. Properties of small things i. Surface to volume ratio affects cell shape and size.
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