Experiment 6 - Experiment 6 Introduction to Spectroscopy...

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Experiment 6: Introduction to Spectroscopy Jonathan Brate January 26, 2009 Monday 2:00-5:00 Mr. Padolik Introduction: This experiment was performed in order to achieve all the objectives listed on page 6-4 in the Lab Book. One objective was to measure the emission spectrum of the fluorescent lights in the laboratory and correlate the spectrum with the colors observed visually. This was done by observing the light with a spectroscope and using it to match up what wavelength each color occurs at. Another objective was to measure the absorption of visible light by a solution of an absorbing substance and examine how the intensity plots result in the calculated absorption spectrum. This was accomplished by examining how much light is absorbed by a given substance with water serving as the reference signal. A spectrometer was used to measure the light, and the MeasureNet system was used to record and print off a plot of the results. A third objective was to prepare a plot of absorbance vs. concentration for a series of solutions and determine the absorptivity of the absorbing species at that wavelength. This was accomplished by recording the absorptivity of the substance and then recording the absorptivity of two diluted samples of the same substance, placing all the graphs on the same printout. Next, the class results were combined to produce a Beer’s Law plot containing all three substances, and used to determine the molar absorptivity of each at the wavelength chosen. Lastly, the
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absorption spectrum of an unknown solution was measured and used to determine the concentration of the solution.
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