Exam3AKEYFall 2007

Exam3AKEYFall 2007 - BBMB 301 Survey of Biochemistry Fall...

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BBMB 301 – Survey of Biochemistry -- Fall 2007 Exam #3 -- Version A Student Name: ______________ KEY ___________ Student I.D. # ______________________________ For 2 points, Write your full name in the upper left hand corner of the back page and the first initial of your last name in the upper right hand corner of the back page. Use only the top inch of the page! Part A – 50 points 1. (10 points) Define any 5 of the following 7 terms: chiral center C atom bonded to 4 unique groups lactose intolerance inability to produce lactase enzyme beyond the childhood years, so cannot digest milk sugar histones basic proteins complexed with DNA in chromatin nucleoside base + sugar alkylating agents chemicals that modify bases by adding alkyl groups, causing mispairing intron sequence on primary transcript that is removed prior to translation of mRNA polyribosome multiple ribosomes on single mRNA 2. (2 points) Complete the statement below by filling in the blanks. In humans, the H antigen is attached to red blood cells. The H antigen contains a preliminary trisaccharide unit of N-acteylglucosamine, galactose, and fucose. In individuals of blood type _O_ _ , this trisaccharide unit is not altered, but in individuals of type _ AB_, two different forms of the enzyme N-acetylgalactosylaminyltransferase (GT) with different catalytic properties add an additional (different) monosaccharide to the trisaccharide unit. 3. (8 points) Complete the following table. Name of Structure(s) Description of Structure maltose Disaccharide composed of glucose monomers linked by α (1 4) sucrose Disaccharide composed of glucose and fructose linked by α , β (1 2) peptidoglycans Polysaccharide found in bacterial cell walls, has peptide cross-links chitin Polysaccharide in exoskeletons containing N-acetylglucosamine chondroitin sulfate hylauronic acid 2 Polysaccharides in synovial fluid and extracellular matrix that are heteropolymers of N-acetylglucosamine and glucuronic acid glycogen Polysaccharide with glucose monomers linked by α (1 4) with α (1 6) branching; found in animals -- not in plants lactose Disaccharide composed of galactose and glucose linked by β (1 4) 1
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3. (4 points) DNA denaturation a. How is DNA denaturation (dsDNA ssDNA) caused by heating identified (monitored) in the laboratory? ssDNA has increased absorbance in the UV range hyperchromic shift / by UV spectroscopy b. DNA sample 1 has a higher melting temperature (T m ) than does DNA sample 2. What does that tell us about the base compositions of the two DNAs? Sample 1 has a higher G:C content than does Sample 2
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This test prep was uploaded on 04/06/2008 for the course BBMB 301 taught by Professor Girton during the Fall '07 term at Iowa State.

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Exam3AKEYFall 2007 - BBMB 301 Survey of Biochemistry Fall...

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