Exam4AKEY - BBMB 301 Survey of Biochemistry Fall 2007...

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BBMB 301 – Survey of Biochemistry -- Fall 2007 Exam #4-- Version A KEY Student Name: _____________________________ Student I.D. # ______________________________ For 2 points, Write your full name in the upper left hand corner of the back page and the first initial of your last name in the upper right hand corner of the back page. Use only the top inch of the page! Part A – Short Answer 1. (10 points) Define any 5 of the following terms: (remember, only the first 5 answers will be graded) recombinant DNA DNA molecule formed by uniting two DNA molecules from different sources vector DNA (usually plasmid or virus) that is capable of replicating autonomously and carrying an insert or foreign DNA catabolism metabolic pathways that involve the degradation of complex molecules to simpler ones, characterized by converging, exergonic reactions dehydrogenases enzymes that catalyze oxidation-reduction reactions often using NAD+/NADH as coenzymes feedback inhibition the product of the reaction causes the enzyme to be less active substrate-level phosphorylation ATP is produced by transfer of a phosphate group from another high energy molecule such as phosphoenol pyruvate mitochondrion organelles in aerobic cells that are the sites of electron transport and oxidative phosphorylation glyoxysomes organelles found in plants that allow them to use acetate and fatty acids as an energy source in a pathway called the glyoxylate pathway 2. (6 points) DNA Cloning Briefly outline the steps involved in a basic DNA cloning experiment using genomic DNA from human liver as the insert DNA, a plasmid vector such as pBR322, and E. coli as the host organism. 1. Obtain genomic DNA and cut it with a restriction endonuclease such as BamH1 that will also cut the vector. 2. Cut the vector (plasmid) DNA with the same restriction endonuclease 3. Mix the DNAs together and add DNA ligase. 4. Prepare transformation competent E. coli cells and perform the transformation procedure. 5. Plate the transformation mixture on antibiotic containing medium (eg. Ampicillin) that will only allow the transformants to grow. 6. Screen the transformed cells to see if they have recombinant DNA (rather than vector only). Many plasmids allow for blue/white colony screening but pBR322 does not. 1
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. (4 points) Transgenic Organisms or Products List 4 examples of useful transgenic plants or animals or proteins that are made using recombinant DNA technology .
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