Wk5_Protocol

Wk5_Protocol - Protocol: 1. Grow bacteria overnight in 10...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Protocol: 1. Grow bacteria overnight in 10 mL of the appropriate medium. (This will be done for you, but WHAT is the “appropriate” medium?). 2. Take 6 sterile micro tubes and add 1 mL of the bacteria into each tube and spin in the microfuge for 3 min. Remove the supernatant and dispose into empty beaker (save on ice). **Yes, you will have leftover bacteria; put it in the biohazard waste. 3. Resuspend pellet in 80 μL of 20% w/v sucrose, 0.03 M Tris-HCl pH 8.0. Add 20 μL of 5 mM sodium EDTA (pH 8.0). Mix by tapping on the bottom of the tubes and shake 10 min at room temperature. 4. Pellet cells in microfuge for 3 min. 5. Remove supernatant from each sample using a pipet, being careful not to disturb the pellet, and transfer to a SINGLE labeled micro tube. Store sample on ice. 6. Resuspend pellet in 100 μL of 50 mM sodium phosphate buffer and shake on ice for 10 min. 7. Pellet cells in microfuge for 3 min. 8. Transfer the supernatant of each sample into a second labeled micro tube.
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 10/24/2011 for the course HLTH 100 taught by Professor Jeleciamiller during the Fall '11 term at Emory.

Page1 / 2

Wk5_Protocol - Protocol: 1. Grow bacteria overnight in 10...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online