Liverlabreport - Liver Lab Deepika Mukhara 2nd period(snap Introduction Rationale The purpose of this experiment was to determine the effect of

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Liver Lab Deepika Mukhara 2nd period (snap)
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Introduction Rationale The purpose of this experiment was to determine the effect of temperature, acidity, and concentration on catalase reactions. Catalase is important because it promotes the decomposition of hydrogen peroxide (H 2 O 2 ). Hydrogen peroxide is a by-product of cell metabolism that is toxic to cells. An experimental hypothesis is if the temperature, concentration, and acidity are higher, then the catalase will have increased activity. The reaction produces oxygen. Therefore, measuring the amount of air (which increases as more oxygen is produced and allowed to go into the tube) is a valid way to measure the amount of oxygen produced during the reaction. Experimental Design One of the independent variable was temperature with three levels; 5°C, 22°C, and 37°C. Six trials at all levels and conducted. For the independent variable of concentration, the three levels were 100%, 50%, and 25%. Six trials at all levels were conducted. The 100% concentration level was the control in this instance. For the independent variable of acidity, the three levels were pH levels of four, seven, and ten. Six trials for all levels were conducted. The level with the pH of seven was the control in this instance. The dependent variable was catalase activity. Catalase activity was measured by the rate of oxygen gas evolved in milliliters per thirty seconds. The constants are the hydrogen peroxide (three percent solution), the amount poured into each test container, the disks, the reaction chamber and the water in the pan.
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Methods Four catalase soaked filter paper disks in the interior wall of the reaction chamber. Ten milliliters of the hydrogen peroxide solution at room temperature (22 degrees Celsius) was poured into the chamber with none of the solution touching the disks. A stopper was tightly placed over the chamber. A pan was filled with water. A 50mL graduated cylinder was placed in the pan so that it filled with water completely and stayed submerged. Making certain the side with the disks were on top, the reaction chamber was placed in the pan of water. The graduated cylinder was placed into a position so that the mouth lay directly over the dropping pipet extended from the reaction chamber. The reaction chamber was rotated 180 degrees so that the sides with the disks came in contact with the hydrogen peroxide solution. The gas solution was
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This note was uploaded on 10/23/2011 for the course BIOLOGY 1819287 taught by Professor Delcerro during the Spring '11 term at Thomas Jefferson School of Law.

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Liverlabreport - Liver Lab Deepika Mukhara 2nd period(snap Introduction Rationale The purpose of this experiment was to determine the effect of

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