215-Lab4B-Yeast_ONs-2

215-Lab4B-Yeast_ONs-2 - Lab 4B Setting up Yeast ON Cultures...

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4B-1 Lab #4B Yeast ONs You will need to come into the lab on Monday Oct. 4, between 7:00 - 9:00 AM or 11:00 AM to 1:00 PM to look at your plates and set up cultures. Background: Next week you will perform a plasmid rescue protocol to purify the plasmid carrying the mutant Hst1:Sir2 chimera from yeast. You will need to pick two of your four mutants to set up fresh ON cultures of your mutants to obtain enough cells to be able to purify the plasmid DNA in the next lab. i) Examine your plates and answer the following questions: a) Does the growth curve (dilution series) of each strain look the same on the SD- leu plate? What does this tell you? b) Is there a difference in the growth curves of the strain on the SD- leu, trp plates? What does this tell you? c) What are the fold differences in the growth curves? d) Choose two of your four mutants that you will examine further in this course. Which two mutants did you pick and why? Note:
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215-Lab4B-Yeast_ONs-2 - Lab 4B Setting up Yeast ON Cultures...

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