Chapter9Biotechnology

Chapter9Biotechnology - Lecture 17 - Genetics Biotechnology...

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Lecture 17 - Genetics Biotechnology
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Learning Objectives Describe how restriction digestion + ligation are used to “cut and paste” fragments of DNA. Describe how DNA fragments can be separated based on size. Understand how the presence or absence of a DNA fragment containing a specific sequence can be determined in any sample of DNA. Understand how mutagenic probes can be used as primers to introduce specific mutations into DNA. List the steps involved in creating a DNA library from a particular organism. Explain how to screen a DNA library for a particular clone. Explain the use of a reporter gene to measure the transcription rate of another gene. Describe the procedure used in a Southern blot, and understand why Southern blots are done. List the steps in a polymerase chain reaction. Explain how PCR and Southern blotting can be used to diagnose infectious and genetic diseases. List the steps involved in cloning a gene into a bacterium. Explain to a friend who is not into science what is involved in making transgenic organisms. List two specific examples of genetically modified organisms. Explain how a microarray experiment is done. Understand how a microarray experiment is used to identify transcription patterns of many
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Chapter9Biotechnology - Lecture 17 - Genetics Biotechnology...

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