sg-Q#10 - a) Endospore Stain b) Flagella Stain c) Capsular...

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Study Guide for Quiz #1 Week 1 Lab 1. Know how to calculate total magnification. What is the magnification of the ocular? 2. Define resolving power. 3. What is the purpose of using immersion oil? 4. List the function for the following parts of the microscope. Ocular: Condenser: Iris diaphragm: Revolving nosepiece: Coarse adjustment: Fine adjustment: 5. Resolution is a function of the ___________ and ___________________. 6. List 3 ways to increase resolution. *Be able to differentiate the function of the condenser & diaphragm.
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Week 2 Lab 1. What charge do acidic dyes have? What charge do basic dyes have? 2. What charge does the cell wall of bacteria have? 3. What are the advantages of a wet mount? What are the advantages of stained preparation? What is one disadvantage of stained preparation?
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4. List the characteristics of the different types of stains. l) Simple Stain 2) Differential Stains a) Gram Stain b) Acid-Fast Stain (Ziehl-Neelsen Stain) 3) Structural Stains
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Unformatted text preview: a) Endospore Stain b) Flagella Stain c) Capsular Stain 4) Negative Stain 5. Name 3 possible capsular material. 6. What is another term for capsule? 7. What is the purpose of using a modant in the Gram stain? What is the purpose of using a decolorizer in the Gram stain? 8. Which technique(s) (wet mount preparation, hanging drop preparation, or stained preparation) can be used to study bacterial size, shape, and motility? 9. Read the handout, "BacLight Bacterial Gram Stain". Week 3 Lab 1. Know the common features of bacteria, protozoa, algae, and fungi and know which kingdom they belong to. 2. Know the differences b/t prokaryotic & eukaryotic cells. 3. Name 2 differences b/t yeast and mold. 4. Name 2 purposes of single colony isolation. 5. Single colony isolation is used to separate one species from another. What 3 differences is this technique based on? 6. What you need to know in order to figure out a bacterial titer? What is the bacterial titer (Lab 4)?...
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This note was uploaded on 10/24/2011 for the course BIOL 221 taught by Professor Walter during the Fall '08 term at Purdue University-West Lafayette.

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sg-Q#10 - a) Endospore Stain b) Flagella Stain c) Capsular...

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