Biology 313, Principles of Genetics
Due Monday March 10, 2008
1) (3 pts) (1A) How are heterogeneous nuclear RNA (hnRNA) and messenger RNA (mRNA)
hnRNA is the primary transcript that is processed into mRNA; hnRNA is pre-mRNA; hnRNA
is processed into mRNA.
(1B) Where in the cell would each type of RNA be found?
hnRNA - nucleus
(1C) List the 3 major processing events required to make mRNA?
Addition of 5’ cap
Addition of poly-A tail
Splicing of introns and ligation of exons
2) (4.5 pts) The nontemplate strand of DNA has the following base sequence:
(2A) Underline in the above sequence an important
-acting promoter element and provide its
-10 region or Pribnow box; also accepted TATA or Goldberg-Hogness box.
(2B) Find the open reading frame and write the corresponding mRNA sequence starting with the 5'
The first START codon (ATG) is shaded, which sets the reading frame.
Every 3 bases are
read until the 1
in-frame STOP codon (underlined) is reached.