lecture 5 - Page 1 Pathophysiology 05 • JP Advis DVM Ph.D...

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Unformatted text preview: Page 1 Pathophysiology 05 • JP Advis DVM, Ph.D. Bartlett Hall, Animal Sciences, Cook, 932 - 9240, [email protected] • Course website: rci.rutgers.edu/~advis • Lectures, tests, grades, office hours, textbook, • Material to be covered: • About lecture slides: • There are not intended to be the sole source for studying the course material !!!!!!!!!!!!!!!! • Slides are good to review the course material after you have study your course textbook • Slides are a good indicator of the relative importance of lecture topics (see slide # per topic • Group slides by titles when using them to review course material. Match lectures and text. Lectures 1-2: Introduction to Pathophysiology (2) Lectures 3-4: Mechanisms of Self-Defense and Stress (2) Lectures 5-8: Endocrine and Nervous System Dysfunctions (4) Lecture 9: Alterations of Skeletal Muscle Function (1) REVIEW AND TEST #1 Lectures 12-18: Cardiovascular, Respiratory and Renal Dysfunctions (7) REVIEW AND TEST #2 Lectures 21-24: Alterations of Digestive Function and Intermediary Metabolism (4) Lectures 25-26: Alterations of the Reproductive System (2) REVIEW AND TEST #3 Endocrinology – the basics METHODS OF HORMONE MEASUREMENTS: a) Radioimmunoassay (RIA). An immunologic technique in which known amounts of Ab and radio-labeled hormone are put in an assay tube with the unlabeled hormone. b) Enzyme – Linked Immunosorbent Assay (ELISA). Similar to RIA but easier to run and less expensive. Instead of radio-labeled hormone, enzyme labeled hormone is used. c) Bioassay. Uses graded doses of hormone in a reference preparation and comparison of results with an unknown sample. Used in early research rather than in clinical labs. Bioassay vs Ab based assays. Basic techniques to assess blood levels of hormones Hormone assays RIA, ELISA, bioassays Structure - Function hypothalamic - pituitary thyroid and parathyroid endocrine pancreas and adrenal glands Action Mechanisms hormone release, transport and hormone action Alterations in General hyper and hypo functions, primary and secondary, diagnostic approaches Central Alterations (e.g) hypothalamus, anterior and posterior pituitary Peripheral Alterations (e.g.) thyroid, parathyroid, pancreas, adrenals Page 2 Endocrinology – the basics Competing reactions that form the basis of the RIA. A.- Labeled hormone (H, shown in red) competes with hormone in a biological sample (green H) for a limited amount of antibodies (Ab). As the concentration of hormone in the biological sample rises (rows 1,2, and 3) decreasing amounts of labeled hormone appear in the hormone-antibody (H-Ab) complex and the ratio of bound / free labeled hormone (B/F) decreases B.- A typical standard curve used to estimate the amount of hormone in biological sample. A B/F ratio of 50% corresponds to 12 ng / ml in this example....
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This note was uploaded on 10/25/2011 for the course ANIMALSCIE 4xx taught by Professor Advis during the Spring '11 term at Rutgers.

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lecture 5 - Page 1 Pathophysiology 05 • JP Advis DVM Ph.D...

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