Exam 2-Study Guide - BiochemExam2StudyGuide 00:17

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Biochem-Exam 2 Study Guide 00:17 1. True/ False statements about restriction enzymes binds to target DNA, cuts DNA at specific target sequence(restriction sites);  “blunt ends” Restriction enzyme cuts directly across DNA helix, “sticky ends”- restriction enzyme cuts the DNA helix staggered;  2. Child usually receives 23 chromosomes from each parent 3. Child inherits 3 copies of any chromosome, what disease is this? Down’s Syndrome 4. MRNA  U instead of T, mRNA splicing 5. Why is folding important in protein producing  incorrect folding of proteins can lead to memory loss and many diseases such as  Alzheimer’s, Huntington’s and Parkinson’s disease 6. Recombinant plasmid Vector that carries the human DNA in cloning, the recombinant plasmid is cloned  in bacteria, each bacteria cell receives one recombinant DNA plasmid. 7. DNA ligase  ???
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8. Why do you wear a lead apron in a dentist office Protects you from harmful radiation waves 9. Cystic fibrosis  30,000 diagnosed each year, 12 million carriers in the US, carriers do not have the  disease they inherit one CF gene (c)  and one CFTR gene(C) (CF transmembrane  conductance regulator), children who have CF inherit both cc genes from parents If both parents are carriers of the CFTR gene there is a 25% chance a baby will  inherit CF, however if only parent has CFTR gene 50% chance the baby will be a  carrier but will not inherit CF 10. Genetic testing can show what/ can be used for which diseases… Amylotropic Lateral Sclerosis (ALS), Alzheimer’s, Parkinson’s, Huntington’s,  Down’s Syndrome(chromosome karyotype testing), Cystic Fibrosis (DNA  sequencing), heart disease, cancer, PKU, sickle-cell,Tay-Sach’s, Klinefelter  (chromosome karyotype)  11. A karyotype is used to pick out                      numbers  karyotype is used to pick out abnormal chromosome numbers or abnormal  chromosome shape 12. You and I have what percent of DNA in common?
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99.9%
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This document was uploaded on 10/27/2011 for the course PORTUGUESE 312 at UMass (Amherst).

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Exam 2-Study Guide - BiochemExam2StudyGuide 00:17

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