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Jan27_Kukor_Phylogeny

Jan27_Kukor_Phylogeny - Environmental Pollution...

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Environmental & Pollution Microbiology Spring 2010 Phylogeny and Microbial Diversity [mostly from Brock chapter 11 in 11 th edition & chapter 14 in 12 th edition] (1.) Evolutionary chronometers (A.) evolutionary distances evaluated from substitutions in homologous molecules -- a direct reflection of mutational changes (B.) Use of rRNA -- universally distributed, functionally homologous, positions can be aligned, rate of substitution directly related to evolutionary distance. (Other useful chronometers are ATPase and RecA)
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(C.) Review structure of ribosome (i.) Subunits & RNA species Prokaryote: 70S overall 30S small subunit 21 proteins 1 RNA (16S) 50S subunit 34 proteins 2 RNAs -- 23S and 5S Eukaryote: 80S overall 40S small subunit 30 proteins 1 RNA (18S) 60S large subunit 50 proteins 3 RNAs -- 28S, 5.8S, 5S
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