Lab 2 materials and methods

Lab 2 materials and methods - Materials and Methods There...

Info icon This preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Materials and Methods There were two parts to this lab, experiment 1 and experiment 2. In experiment 1, the objective was to compare the differences in photosynthetic pigments found in light-grown and dark-grown barley. In order to this, we had to use thin layer chromatography. After familiarizing ourselves with the thin layer chromatography (TLC) spotting process, we had to produce the solution. We started by grinding up 4-8 leaves of cut, dark-grown barley leaves in 3 mL of methanol using a mortar and a pestle for several minutes. After the leaves were sufficiently ground up, we transferred some of the mixture into a microcentrifuge tube. We cleaned out the mortar and pestle and repeated the same grinding process with 2-4 leaves of finely cut light- grown barley. We then centrifuged the two barley extracts for five minutes. After, we started spotting the two extracts onto the TLC strip, 0.5 cm from the bottom. We would spot it once, run air over it to dry out the spot, and spot it again. We spotted the paper twenty times for each
Image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern