Lab 2 materials and methods

Lab 2 materials and methods - Materials and Methods There...

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Materials and Methods There were two parts to this lab, experiment 1 and experiment 2. In experiment 1, the objective was to compare the differences in photosynthetic pigments found in light-grown and dark-grown barley. In order to this, we had to use thin layer chromatography. After familiarizing ourselves with the thin layer chromatography (TLC) spotting process, we had to produce the solution. We started by grinding up 4-8 leaves of cut, dark-grown barley leaves in 3 mL of methanol using a mortar and a pestle for several minutes. After the leaves were sufficiently ground up, we transferred some of the mixture into a microcentrifuge tube. We cleaned out the mortar and pestle and repeated the same grinding process with 2-4 leaves of finely cut light- grown barley. We then centrifuged the two barley extracts for five minutes. After, we started spotting the two extracts onto the TLC strip, 0.5 cm from the bottom. We would spot it once, run air over it to dry out the spot, and spot it again. We spotted the paper twenty times for each
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This note was uploaded on 10/26/2011 for the course LS 2 taught by Professor Pires during the Spring '08 term at UCLA.

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Lab 2 materials and methods - Materials and Methods There...

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