LS2lab3 - Meghana Karmarkar Ramita Sawhney Jasmine Graves...

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Meghana Karmarkar Ramita Sawhney Jasmine Graves LS 2 Discussion 2K Effects of Nicotine on Oxygen Consumption Rate in Goldfish Introduction: Metabolism is the sum of all chemical processes in the body. Anabolic processes synthesize complex molecules, while catabolic processes breakdown these molecules. Our experiment involves studying the metabolism of Goldfish. To measure the metabolic rate, we measured the oxygen consumption rate of Goldfish, since this the bulk of what Goldfish consume (besides Fish food). We chose Goldfish as our model organism because they are poikilothermic; that is, their body temperature changes when the environmental temperature changes. This makes it easier to calculate the metabolic rate. We want to test what the effects of Nicotine will be on oxygen consumption. Nicotine is a drug derived from a family of plants that functions both as a stimulant and a relaxant. It can enter the Central Nervous System where it triggers the release of neurotransmitters related to relaxation. Nicotine also affects the adrenal medulla and triggers the release of epinephrine, associated with stimulation and alertness, as well as an increased metabolism. We conducted our experiment within a small oxygen chamber that has a built-in oxygen probe attached to our computer. This probe allowed us to observe continuous readings of the oxygen concentration of the chamber’s water. Based on this background, our null hypothesis is that Nicotine does not affect the rate of oxygen consumption in Goldfish. Our hypothesis is the Nicotine does have an effect on the rate of oxygen consumption in Goldfish. We chose this hypothesis because we predict that Nicotine will have similar effects on the nervous system of Goldfish as it does on the nervous system of humans. If this is true, then we should observe that the Nicotine will act as either a stimulant or a
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relaxant (or both) on the Goldfish. In either case, we should observe that oxygen consumption will either increase or decrease, thus having an effect on the rate. Materials and Methods: In this experiment, we performed two trials, both of which were exactly the same procedure but were repeated in order to average out the data and to ensure that the first trial was not erroneous. Each trial had two parts: the first part was controlled whereas the second part was the experiment. In the experimental part, one variable was chosen to change the chemical nature of the water for the fish so that we could measure the difference (if any) of the oxygen consumption rate of goldfish under altered environmental conditions. Our group chose nicotine as the variable, so we used 1.5 grams of tobacco infused in fish water to experiment with the goldfish. To determine their oxygen consumption rate, we used an oxygen probe that was attached to the water chamber. The probe is connected to a computer program called LoggerLite, which measures oxygen consumption every 20 seconds for 10 minutes. LoggerLite works by
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This note was uploaded on 10/26/2011 for the course LS 2 taught by Professor Pires during the Spring '08 term at UCLA.

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LS2lab3 - Meghana Karmarkar Ramita Sawhney Jasmine Graves...

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