Unformatted text preview: 6 mL of buffer 5 followed by 10 mL dH2O. Collect the flow though in beaker 1. Then wash the resin with 30mL .5M NaOH collect the flow in beaker 2. Wash the resin with another 10mL dH2O. Lastly, wash the resin with 10mL 20% ethanol. Collect the flow in beaker 1. Waste in the beakers should be disposed in the designated containers. Load several mL 20% ethanol into column. Cap the column, invert it to generate slurry. Pour it in the designated container. Wash the column and lids and return them....
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This note was uploaded on 10/25/2011 for the course ALL 101 taught by Professor All during the Spring '11 term at Cameron University.
- Spring '11